| CONFIGURATION | |
|---|---|
| Kit contents | StellarScript® HT RT (200 U/µL), 10X RT Reaction Buffer, 10 mM dNTP Mix, 100 mM DTT |
| Unit definition | One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly(A)/oligo (dT) as a substrate |
| Enzyme storage buffer formulation | 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.1% Tween 20, 50% glycerol |
| 10X RT Reaction Buffer formulation |
500 mM Tris-HCl (pH 8.3), 750 mM KCl, 30 mM MgCl₂ |
| QUALITY CONTROL | |
|---|---|
| Purity (SDS-PAGE) | >99% |
| dsDNA exonuclease | <1% released |
| ssDNA exonuclease | <1% released |
| DNA contamination (E. coli, mammalian, library) |
>10 copies |
| Phosphatase assay | >1% release |
| Endonuclease | Not detectable |
| Nonspecific RNase | Not detectable |
| SHIPPING AND HANDLING | |
|---|---|
| Shipping conditions | Ice packs |
| Storage | -20°C ± 5°C |
| Shelf life | 12 months |