Taq Highlights

Taq DNA polymerase is a thermostable DNA polymerase (DNAP) that catalyzes 5′ → 3′ DNA synthesis. Taq DNA polymerase has 5′ → 3′ exonuclease activity making it suitable for probe digestion and lacks 3′ → 5′ exonuclease activity.

  • Strong terminal transferase activity enables A-tailing to support AT ligation chemistries and cloning
  • Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price-point
  • Custom formats, including high concentration, support lyophilization applications
  • Highly stringent enzyme manufacturing ensures quality performance across lots


  • 3′ dA-tailing (A-tailing)
  • NGS library preparation
  • PCR amplification (fragments ≤ 5kb)
  • Cloning


Engineered Hot-start Taq DNA Polymerases

Highly active and inhibitor-tolerant polymerases for stringent PCR and qPCR applications


QC Specifications
Description Specification
Protein Purity Assay ≥ 99%
dsDNA Exonuclease Assay* <1% released
ssDNA Exonuclease Assay* <1% released
DNA contamination Assay (E. coli, mammalian, library)** < 10 copies
Phosphatase Contamination Assay* < 1% released
Endonuclease Contamination Assay* Not detectable

*As assessed using 37 U of enzyme input per assay.
**As assessed using 5 U of enzyme input per assay.


Unit definition: One unit of Taq DNA polymerase is the amount of enzyme that will incorporate 10 nmol of dNTP into activated calf thymus DNA in 30 minutes at 75°C

Reaction conditions:
20 mM Tris-HCl, pH 8.3
40 mM KCl
0.04% Tween
1 – 4 mM MgCl₂

Storage buffer: 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.5% Tween

Heat inactivation: No

Molecular weight: 93.9 kDa

5’ – 3’ Exonuclease activity: Yes
3’ – 5’ Exonuclease activity: No
Strand Displacement activity: No
Fidelity: Low
Processivity: Moderate