Taq Highlights
Taq DNA polymerase is a thermostable DNA polymerase (DNAP) that catalyzes 5′ → 3′ DNA synthesis. Taq DNA polymerase has 5′ → 3′ exonuclease activity making it suitable for probe digestion and lacks 3′ → 5′ exonuclease activity.
- Strong terminal transferase activity enables A-tailing to support AT ligation chemistries and cloning
- Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price-point
- Custom formats, including high concentration, support lyophilization applications
- Highly stringent enzyme manufacturing ensures quality performance across lots
Applications
- 3′ dA-tailing (A-tailing)
- NGS library preparation
- PCR amplification (fragments ≤ 5kb)
- Cloning
COMING SOON
Engineered Hot-start Taq DNA Polymerases
Highly active and inhibitor-tolerant polymerases for stringent PCR and qPCR applications
EARLY ACCESS SAMPLES AVAILABLE NOW!
QC Specifications
| Description | Specification |
|---|---|
| Protein Purity Assay | ≥ 99% |
| dsDNA Exonuclease Assay* | <1% released |
| ssDNA Exonuclease Assay* | <1% released |
| DNA contamination Assay (E. coli, mammalian, library)** | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Endonuclease Contamination Assay* | Not detectable |
*As assessed using 37 U of enzyme input per assay.
**As assessed using 5 U of enzyme input per assay.
Properties
Unit definition: One unit of Taq DNA polymerase is the amount of enzyme that will incorporate 10 nmol of dNTP into activated calf thymus DNA in 30 minutes at 75°C
Reaction conditions:
20 mM Tris-HCl, pH 8.3
40 mM KCl
0.04% Tween
1 – 4 mM MgCl₂
Storage buffer: 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.5% Tween
Heat inactivation: No
Molecular weight: 93.9 kDa
5’ – 3’ Exonuclease activity: Yes
3’ – 5’ Exonuclease activity: No
Strand Displacement activity: No
Fidelity: Low
Processivity: Moderate