RNase INHIBITOR

PRODUCTS

Protect what’s precious

RNase Inhibitor exhibits high affinity, non-competitive binding of RNases A, B, and C at a 1:1 ratio, enabling high-quality cDNA synthesis from low-quality RNA samples. The absence of two cysteines present in human and porcine RNase inhibitors make this murine version highly suitable for low-DTT applications.

  • Prevent RNA degradation in RT-qPCR, single cell, and single nuclei sequencing workflows at temperatures up to 55°C
  • Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price-point
  • Improve performance in low-DTT settings with a murine RNase inhibitor that better resists oxidation
  • Custom formats, including high concentration, support lyophilization applications
  • Highly stringent enzyme manufacturing ensures quality performance across lots

Applications

  • Nuclei isolation
  • RT-PCR and RT-qPCR
  • cDNA synthesis
  • Applications where maintaining RNA integrity is critical
  • Single-cell RNA sequencing
  • Bulk RNA-sequencing
  • Cell-free cloning
  • In vitro synthesis (IVT)

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Key Performance Data

Safeguard RNA assay performance

Ribonucleases (RNases) are ubiquitous and can have significant and detrimental impacts on assay performance and sensitivity. Incorporating an RNase inhibitor to prevent RNA degradation ensures reliability and accuracy of experimental results – particularly for studies and assays focused on pathogen detection, gene expression, RNA stability, and other RNA-dependent processes.

QC Specifications
Description Specification
Protein Purity Assay ≥ 97%
dsDNA Exonuclease Assay* <1% released
ssDNA Exonuclease Assay* <1% released
DNA contamination Assay (E. coli, mammalian, library)* < 10 copies
Phosphatase Contamination Assay* < 1% released
Endonuclease Contamination Assay* Not detectable
Nonspecific RNase* Not detectable

*As assessed using 450 U of protein input per assay.

Properties

Unit definition: One unit of RNase inhibitor is defined as the amount of RNase Inhibitor required to inhibit activity of 0.375 ng of RNase A by ≥ 95%

Reaction conditions:
Active at temperatures ≤ 55°C

Storage buffer: 20 mM HEPES-KOH, 0.1 mM EDTA, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.6

Heat inactivation: 70°C for 20 min

Molecular weight: 50.6 kDa