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Precision Enzymes to fuel cutting-edge applications
The StellarScript™ Reverse Transcriptase (RT) portfolio features three M-MLV variants with reduced RNase H activity and enhanced processivity, activity, thermostability, and inhibitor tolerance in comparison to wild-type enzyme. Kits contain all required reagents for cDNA synthesis except primers. The portfolio includes:
- StellarScript for template switching and robust performance
- StellarScript HT for increased thermostability and inhibitor tolerance
- StellarScript HT+ for template switching and the highest thermostability and inhibitor tolerance
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Tailored reverse transcriptases to meet your application needs
Property | Application | |||||||||
Optimal Temp |
Thermal Stability |
Inhibitor Tolerance |
Template Switching |
RT-qPCR/ qRT-PCR |
RT-PCR | RNA-seq | scRNA-seq | 5′ RACE | 3′ RACE | |
StellarScript | 42˚C | + | + | + | + | + | + | + | + | + |
StellarScript HT | 42 – 50˚C | ++ | ++ | – | ++ | ++ | + | – | – | + |
StellarScript HT+ | 42 – 65˚C | +++ | +++ | + | +++ | +++ | + | + | + | + |
Table 1. Overview of StellarScript portfolio enzyme properties and relevant applications. “+” signs indicate strength of a property or fit of an application for a specific enzyme.
Improve specificity with increased thermostability
The high degree of secondary structure in many RNA species, such as high-GC transcripts, can make full-length generation of cDNA difficult and decrease overall assay sensitivity. Increased reverse transcription temperatures relax secondary structure, but this approach requires a highly thermostable reverse transcriptase. The StellarScript portfolio contains variants with a wide range of optimal reverse transcription temperatures to increase cDNA product specificity.
High-quality performance across a wide range of RNA inputs and qualities
The StellarScript portfolio delivers excellent performance across a wide range of RNA inputs and qualities, including highly damaged FFPE material and inputs as low as 10 pg. This provides broad application and sample type compatibility.
Want more information? Visit our other StellarScript portfolio product pages:
StellarScript
Robust cDNA synthesis from as little as 10 pg with template switching activity.
StellarScript HT
Improved thermostability and inhibitor tolerance improves compatibility with challenging applications.
Stellarscript HT+
Excellent thermostability and inhibitor tolerance, paired with template switching capability, delivers high-quality performance with a variety of samples, targets, and applications.
Coming soon: High-purity RNase Inhibitor
Protect what’s precious for your single cell, single nuclei, and RT-qPCR workflows.