STELLARSCRIPT REVERSE TRANSCRIPTASE PORTFOLIO

PRODUCTS

Precision Enzymes to fuel cutting-edge applications

The StellarScript™ Reverse Transcriptase (RT) portfolio features three M-MLV variants with reduced RNase H activity and enhanced processivity, activity, thermostability, and inhibitor tolerance in comparison to wild-type enzyme. Kits contain all required reagents for cDNA synthesis except primers. The portfolio includes:

Tailored reverse transcriptases to meet your application needs

  Property Application
  Optimal
Temp
Thermal
Stability
Inhibitor
Tolerance
Template
Switching
RT-qPCR/
qRT-PCR
RT-PCR RNA-seq scRNA-seq 5′ RACE 3′ RACE
StellarScript 42˚C + + + + + + + + +
StellarScript HT 42 – 50˚C ++ ++ ++ ++ + +
StellarScript HT+ 42 – 65˚C +++ +++ + +++ +++ + + + +

Table 1. Overview of StellarScript portfolio enzyme properties and relevant applications. “+” signs indicate strength of a property or fit of an application for a specific enzyme.

Improve specificity with increased thermostability

The high degree of secondary structure in many RNA species, such as high-GC transcripts, can make full-length generation of cDNA difficult and decrease overall assay sensitivity. Increased reverse transcription temperatures relax secondary structure, but this approach requires a highly thermostable reverse transcriptase. The StellarScript portfolio contains variants with a wide range of optimal reverse transcription temperatures to increase cDNA product specificity.

High-quality performance across a wide range of RNA inputs and qualities

The StellarScript portfolio delivers excellent performance across a wide range of RNA inputs and qualities, including highly damaged FFPE material and inputs as low as 10 pg. This provides broad application and sample type compatibility.

Learn more about:
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StellarScript

Robust cDNA synthesis from as little as 10 pg with template switching activity.

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StellarScript HT

Improved thermostability and inhibitor tolerance improves compatibility with challenging applications.

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Stellarscript HT+

Excellent thermostability and inhibitor tolerance, paired with template switching capability, delivers high-quality performance with a variety of samples, targets, and applications.

Ready to test?

Contact sales@watchmakergenomics.com