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Cover your bases (no matter the sample)
While DNA library preparation has become commonplace in most genomics labs, many challenges related to performance, reliability, and scalability remain — particularly when DNA template is limited or of variable quality. Watchmaker offers two streamlined and flexible workflows that address all sample types while yielding high conversion rates, reduced bias and artifacts, and reproducible results.
- Watchmaker DNA Library Prep Kits with Fragmentation combine accuracy and scalability for highly sensitive clinical and translational applications, including FFPE analysis
- Watchmaker DNA Library Prep Kits deliver improved coverage and sequencing economy with fragmented DNA, including cfDNA from liquid biopsies
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Key Highlights:
- Reduce sequence bias and artifacts to improve accuracy and sensitivity
- Improve coverage of low-frequency variants in cfDNA from liquid biopsies
- Rapidly prepare libraries in under 2 hours with as little as 500 pg
- Robust and consistent enzymatic fragmentation simplifies library prep from FFPE and low-inputs
Simple, automatable workflows for all sample types
Product comparison
| Watchmaker Library DNA Library Prep Kit with Fragmentation |
Watchmaker Library DNA Library Prep Kit | |
|---|---|---|
| Input | dsDNA that requires fragmentation | dsDNA that is already sheared or does not require fragmentation |
| Input range | < 1 ng to 500 ng | 500 pg – 1 µg |
| Library prep time (excluding PCR) |
90 – 140 min (including enzymatic fragmentation) |
115 – 170 min (from fragmented input) |
| Adapter compatibility | Full-length UDI or CDI adapters “Stubby” adapters Adapters with/without UMIs |
Full-length UDI or CDI adapters “Stubby” adapters Adapters with/without UMIs |
| Supports PCR-free workflows | Yes | Yes |
| Single tube library prep | Yes | Yes |
| Tunable size selection | Optional | Optional |
| Automation-friendly workflow | Yes | Yes |
Sample type compatibility
| Watchmaker Library DNA Library Prep Kit with Fragmentation |
Watchmaker Library DNA Library Prep Kit | |
|---|---|---|
| FFPE DNA | X | X |
| Genomic DNA | X | X |
| Plasmids and vectors | X | X |
| Long amplicons | X | X |
| cfDNA | X | |
| ChIP DNA | X | |
| Bisulfite-converted DNA* | X | |
| Short PCR products | X | |
*In combination with Equinox Uracil Tolerant Library Amplification Kits.
Application fit
| Watchmaker Library DNA Library Prep Kit with Fragmentation |
Watchmaker Library DNA Library Prep Kit | |
|---|---|---|
| Whole genome sequencing (human, microbial) | X | X |
| Targeted sequencing (hyb. capture) | X | X |
| Somatic and germline variant detection | X | X |
| Copy number variation (CNV) analysis | X | X |
| Detection of translocations and other structural variants | X | X |
| Metagenomic analysis | X | X |
| Bulk RNA-seq (from full-length or fragmented cDNA) | X | X |
| Viral sequencing | X | X |
| cfDNA / ctDNA / liquid biopsy analysis | X | |
| Fragmentomics | X | |
| ChIP-seq | X | |
| Methyl-seq* | X | |
*In combination with Equinox Uracil Tolerant Library Amplification Kits.
Learn more about:
Watchmaker RNA Library Prep Kits
Generate stranded, high-complexity RNA-seq libraries in under 3.5 hours with inputs ranging from 0.25 to 100 ng total RNA
Equinox Library Amplification Kits
Ultra-high-fidelity DNA polymerase in an optimized master mix, specifically developed for high-efficiency, low-bias NGS library amplification
Watchmaker DNA Library Prep Kits with Fragmentation
A streamlined single-tube chemistry with reduced bias and artifacts. Ideally suited for PCR-free workflows, ultra-low inputs, and low-quality DNA samples including FFPE