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gp32 Highlights
T4 Gene 32 (gp32) is a single-stranded DNA binding protein (SSBP) that is involved in T4 bacteriophage replication and repair. gp32 binds and stabilizes single-stranded DNA and is utilized as an additive in molecular biology assays to increase DNA yield.
- Increase efficiency and primer specificity during PCR and isothermal amplification
- Enhance yield during RT-PCR
- Custom formats, including high concentration, support lyophilization applications
- Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price point
- Highly stringent enzyme manufacturing ensures quality performance across lots
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Applications
- RT-PCR
- Recombinase polymerase amplification (RPA)
- Whole genome amplification (WGA)
- Helicase-dependent amplification
- Isothermal amplification
- Restriction enzyme digestion
- Electron microscopy
Figure 1. T4 Gene 32 Protein bound to single stranded DNA during helicase-dependent polymerization.
QC Specifications
| Description | Specification |
|---|---|
| Protein Purity Assay | ≥ 99% |
| Exonuclease Assay* | Functional |
| Specific Activity* | 100,000 – 190,000 U/mg |
| DNA contamination Assay (E. coli, mammalian, library)* | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Endonuclease Contamination Assay* | Not detectable |
*As assessed using 25 mg of enzyme input per assay.
Properties
Storage buffer: 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 50% Glycerol
Heat inactivation: 65°C for 20 min
Molecular weight: 33.5 kDa