WATCHMAKER RNA LIBRARY PREP KITS

PRODUCTS

Sensitivity meets speed

Watchmaker RNA Library Prep Kits deliver stranded, high-complexity RNA-seq libraries in under 3.5 hours with inputs ranging from 0.25 to 100 ng total RNA.

The easily automated workflow saves time and reduces consumable use by incorporating fewer cleanup and handling steps. Performance with low-input and degraded samples is improved by the incorporation of a novel, engineered reverse transcriptase.

Key Features and Benefits

  • A novel treatment step improves robustness with challenging FFPE samples
  • The rapid, streamlined workflow generates stranded, high-quality libraries in under 3.5 hours
  • Reduced handling and purification steps enhance automatability
  • A reverse transcriptase engineered specifically for RNA sequencing improves conversion of RNA to cDNA

Applications

  • RNA sequencing with degraded samples, such as FFPE
  • Targeted sequencing protocols employing hybridization capture
  • Gene fusion detection

Streamlined, automatable workflow

Figure 1. A simple, rapid workflow for scalable sample processing.

Figure 1. A simple, rapid workflow for scalable sample processing. The Watchmaker RNA Library Prep Kit combines and shortens reaction steps and has fewer cleanups than other commercially available kits.

Key Performance Data

A solution designed with FFPE samples in mind

FFPE samples are inherently challenging to process due to template damage and residual cross-linking. Watchmaker’s novel FFPE treatment step, paired with a reverse transcriptase engineered for RNA sequencing, provides highly sensitivity performance and makes more clinically relevant samples addressable.

Data shown were generated with ribosomal and globin depletion upstream of library prep. Learn more about the Watchmaker RNA Library Prep Kit with Polaris Depletion.

Improve sensitivity with low-input samples

Template-limited sample types, such as fine needle biopsies, can be difficult to process robustly and reproducibly—limiting the sensitivity of associated assays. The Watchmaker RNA Library Prep Kit generates high-complexity libraries with as little as 0.25 ng of RNA.

Data shown were generated with ribosomal and globin depletion upstream of library prep. Learn more about the Watchmaker RNA Library Prep Kit with Polaris Depletion.