| RNA input range | 0.25 - 100 ng of previously enriched or total RNA |
|---|---|
| Watchmaker RNA Library Prep Kit contents | FFPE Treatment Buffer Frag & Prime Buffer 1st Strand Buffer 1st Strand Enzyme 2nd Strand Enzyme 2nd Strand Buffer Ligation Enzyme Ligation Buffer Equinox Library Amplification Master Mix (2X) P5/P7 Primer Mix (10X) |
| Shipping conditions | Ice packs |
| Storage | -20°C ± 5°C |
| Shelf life | (24 rxn): ≥ 6 months (> 24 rxn): ≥ 12 months |
The StellarScript™ Reverse Transcriptase (RT) portfolio features three M-MLV variants with reduced RNase H activity and enhanced processivity, activity, thermostability, and inhibitor tolerance in comparison to wild-type enzyme.
Sensitive and rapid RNA Library Prep Solutions to address a majority of bulk RNA-seq applications and sample types, including low-input and FFPE, with workflows enabling library prep in under 5 hours. The portfolio includes:
Streamlined and flexible DNA Library Prep Solutions that address all sample types while yielding high conversion rates, reduced bias and artifacts, and reproducible results. The portfolio includes:
Equinox Library Amplification Kits deliver excellent fidelity, uniform sequence coverage, and high library complexity to specifically address the stringent demands of applications such as rare variant detection, circulating cell-free DNA (cfDNA) analysis, single-cell analysis, and hybridization capture.
StellarScript Reverse Transcriptases for reduced RNase H activity and enhanced processivity, activity, thermostability, and inhibitor tolerance.
Streamlined and robust Automation Solutions for NGS Library Prep play a crucial role in improving efficiency, accuracy, and reproducibility. Learn about Watchmaker’s focus and efforts in this critical area.
Watchmaker’s purpose-driven development approach ensures the needs of rigorous and highly sensitive applications are met. Explore data for some key applications:
