STELLARSCRIPT REVERSE TRANSCRIPTASE KITS

PRODUCTS

StellarScript Highlights

  • Increased activity generates cDNA from exceedingly low RNA inputs
  • Template switching activity delivers compatibility with applications such as single-cell RNA-seq and 5’ RACE
  • Enhanced thermostability in comparison with wild-type enzyme improves reverse transcription through RNA secondary structure
  • Kits contain all required reagents for cDNA synthesis, except primers, for simple experimental setup
  • Custom formats available, including those to support lyophilization applications

Applications

  • First strand cDNA synthesis
  • Reverse transcription quantitative PCR
    (RT-qPCR)
  • Reverse transcription PCR (RT-PCR)
  • Primer extension
  • RNA sequencing (RNA-seq)
  • Single cell RNA sequencing (scRNA-seq)
  • 3’ and 5’ Rapid amplification of cDNA ends (RACE)
  • Applications leveraging SuperScript II for reverse transcription

Key Performance Data

Achieve robust performance with low-input amounts

Many applications are limited in the available amount of input RNA, which can impact assay performance. StellarScript generates high-quality cDNA across a wide range of RNA input amounts, delivering sensitive performance with very little RNA template.

Improve specificity with a thermostable RT

StellarScript provides enhanced thermostability in comparison to wild-type M-MLV RT and performs equivalent-to-better in comparison to other commercially available M-MLV RT variants with respect to both cDNA yield and lengths generated at elevated temperatures. This improves performance with RNAs that have high degrees of secondary structure, including viral RNA and those that are GC-rich.

For further enhanced thermostability, see StellarScript HT and StellarScript HT+.

Visit our other StellarScript portfolio product pages:

  Property Application
  Optimal
Temp
Thermal
Stability
Inhibitor
Tolerance
Template
Switching
RT-qPCR/
qRT-PCR
RT-PCR RNA-seq scRNA-seq 5′ RACE 3′ RACE
StellarScript 42˚C + + + + + + + + +
StellarScript HT 42 – 50˚C ++ ++ ++ ++ + +
StellarScript HT+ 42 – 65˚C +++ +++ + +++ +++ + + + +

Table 1. Overview of StellarScript portfolio enzyme properties and relevant applications. “+” signs indicate strength of a property or fit of an application for a specific enzyme.

Ready to test or want to learn about our custom options?
Contact sales@watchmakergenomics.com