STELLARSCRIPT HT REVERSE TRANSCRIPTASE KITS

PRODUCTS

StellarScript HT Highlights

  • Increased thermostability enhances cDNA generation from RNA with a high degree of secondary structure
  • Resistance to common inhibitors provides compatibility with a variety of sample sources and applications
  • Improved activity enables reverse transcription from input amounts as low as 10 pg
  • Kits contain all required reagents for cDNA synthesis, except primers, for simple experimental setup
  • Custom formats available, including those to support lyophilization applications

Applications

  • First strand cDNA synthesis
  • Reverse transcription quantitative
    (RT-qPCR)
  • Reverse transcription PCR (RT-PCR)
  • Primer extension
  • RNA sequencing (RNA-seq)
  • 3’ Rapid amplification of cDNA ends (RACE)
  • Applications leveraging SuperScript III for reverse transcription

Key Performance Data

Overcome secondary structure with a thermostable RT

It can be difficult to generate full-length cDNA from RNAs with a high degree of secondary structure, such GC-rich and viral RNAs. Reverse transcribing at elevated temperatures can relax this secondary structure, as well as reduce nonspecific primer hybridization.

StellarScript HT generates higher cDNA yields and performs equivalently with respect to processivity relative to SuperScript III at elevated temperatures.

For maximum thermostability, see StellarScript HT+.

Increased resistance to common inhibitors

Inhibitors reduce cDNA synthesis efficiency and can adversely impact assay sensitivity. Improved inhibitor tolerance safeguards performance and provides compatibility across a wide range of sample types and sources.

StellarScript HT delivers superior inhibitor tolerance in comparison to StellarScript. For maximum inhibitor tolerance, see StellarScript HT+.

Generate cDNA from vanishingly low RNA inputs

StellarScript HT robustly synthesizes cDNA from a broad range of RNA inputs, including as little as 10 pg. This enhances compatibility with applications where RNA template is limiting.

Visit our other StellarScript portfolio product pages:

  Property Application
  Optimal
Temp
Thermal
Stability
Inhibitor
Tolerance
Template
Switching
RT-qPCR/
qRT-PCR
RT-PCR RNA-seq scRNA-seq 5′ RACE 3′ RACE
StellarScript 42˚C + + + + + + + + +
StellarScript HT 42 – 50˚C ++ ++ ++ ++ + +
StellarScript HT+ 42 – 65˚C +++ +++ + +++ +++ + + + +

Table 1. Overview of StellarScript portfolio enzyme properties and relevant applications. “+” signs indicate strength of a property or fit of an application for a specific enzyme.

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