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T4 DNA Polymerase Highlights
T4 DNA polymerase is a mesophilic DNA polymerase that catalyzes 5′ → 3′ synthesis of DNA and has 3′ → 5′ exonuclease (proofreading) activity, but no 5′ → 3′ exonuclease activity. It removes 3′ overhangs and fills-in 5′ overhangs to form blunt ends.
- Efficiently blunt-end double-stranded DNA
- Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price-point
- Custom formats, including high concentration, support lyophilization applications
- Highly stringent enzyme manufacturing ensures quality performance across lots
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Applications
- Gap filling
- Generation of blunt DNA ends (removal of 3′ overhangs and fill in 5′ overhangs)
- Probe labeling via replacement synthesis
ALSO AVAILABLE
High-purity T4 DNA Ligase
Available with standard and rapid ligation buffers for simple optimization
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For assay developers – make realizing your next product easier
- Navigate the complexities of productization with an experienced team who’s as committed to your success as you are
- All aspects of our customization process are designed to serve you with speed, agility, and above all else, a commitment to quality
- Tailored fill volumes, labeling (including white and private label), and packaging designed to your specifications
- All products are manufactured within an ISO 13485:2016-certified QMS (download our certificate)
QC Specifications
| Description | Specification |
|---|---|
| Protein Purity Assay | ≥ 98% |
| Endonuclease Contamination Assay* | Not detectable |
| DNA contamination Assay (E. coli, mammalian, library)* | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Unit Assay* | Pass |
*As assessed using 2 U of enzyme input per assay.
Properties
Unit definition: One unit is defined as the amount of T4 DNA Polymerase that will incorporate 160 nmol of dNTPs into a DNA template in 60 minutes at 37°C
Reaction conditions (materials not included in the kit):
10 mM Tris-HCl, pH 7.9
50 mM NaCl
10 mM MgCl₂
1 mM Dtt
Storage buffer: 1100 mM Potassium Phosphate, pH 6.5, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol
Heat inactivation: 75°C for 20 min
Molecular weight: 103.6 kDa
5′ – 3′ Exonuclease Activity: No
3′ – 5′ Exonuclease Activity: Yes
Strand Displacement Activity: No
Fidelity: Moderate
Processivity: Moderate