STELLARSCRIPT HT REVERSE TRANSCRIPTASE KITS

PRODUCTS

CONFIGURATION

Kit contents StellarScript HT RT (200 U/µL), 10X RT Reaction Buffer, 10 mM dNTP Mix, 100 mM DTT
Unit definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly(A)/oligo (dT) as a substrate
Enzyme storage buffer formulation 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.1% Tween 20, 50% glycerol
10X RT Reaction
Buffer formulation
500 mM Tris-HCl (pH 8.3), 750 mM KCl, 30 mM MgCl₂

QUALITY CONTROL

Purity (SDS-PAGE) >99%
dsDNA exonuclease <1% released
ssDNA exonuclease <1% released
DNA contamination
(E. coli, mammalian, library)
<10 copies
Phosphatase assay <1% release
Endonuclease Not detectable
Nonspecific RNase Not detectable

SHIPPING AND HANDLING

Shipping conditions Ice packs
Storage -20°C ± 5°C
Shelf life 12 months