Sensitivity meets speed
Watchmaker RNA Library Prep Kits deliver stranded, high-complexity RNA-seq libraries in under 3.5 hours with inputs ranging from 0.25 to 100 ng total RNA.
The easily automated workflow saves time and reduces consumable use by incorporating fewer cleanup and handling steps. Performance with low-input and degraded samples is improved by the incorporation of a novel, engineered reverse transcriptase.
Key Features and Benefits
- A novel treatment step improves robustness with challenging FFPE samples
- The rapid, streamlined workflow generates stranded, high-quality libraries in under 3.5 hours
- Reduced handling and purification steps enhance automatability
- A reverse transcriptase engineered specifically for RNA sequencing improves conversion of RNA to cDNA
AVAILABLE NOW
Watchmaker mRNA Library Prep Kit
A rapid and sensitive solution for your gene expression studies.
Applications
- RNA sequencing with degraded samples, such as FFPE
- Targeted sequencing protocols employing hybridization capture
- Gene fusion detection
Streamlined, automatable workflow
Figure 1. A simple, rapid workflow for scalable sample processing. The Watchmaker RNA Library Prep Kit combines and shortens reaction steps and has fewer cleanups than other commercially available kits.
Key Performance Data
A solution designed with FFPE samples in mind
FFPE samples are inherently challenging to process due to template damage and residual cross-linking. Watchmaker’s novel FFPE treatment step, paired with a reverse transcriptase engineered for RNA sequencing, provides highly sensitivity performance and makes more clinically relevant samples addressable.
Data shown were generated with ribosomal and globin depletion upstream of library prep. Learn more about the Watchmaker RNA Library Prep Kit with Polaris Depletion.
Improve sensitivity with low-input samples
Template-limited sample types, such as fine needle biopsies, can be difficult to process robustly and reproducibly—limiting the sensitivity of associated assays. The Watchmaker RNA Library Prep Kit generates high-complexity libraries with as little as 0.25 ng of RNA.
Data shown were generated with ribosomal and globin depletion upstream of library prep. Learn more about the Watchmaker RNA Library Prep Kit with Polaris Depletion.
Accurately quantify gene expression across input amounts
Agreement between high- and low-input libraries with respect to unique genes identified and their abundance is indicative of how well sample complexity is maintained as RNA input amounts decrease. Watchmaker better preserves the gene expression profile as RNA input amount decreases from 500 ng to 10 ng, whereas other commercial solutions result in data distortion with low inputs.
Data shown were generated with ribosomal and globin depletion upstream of library prep. Learn more about the Watchmaker RNA Library Prep Kit with Polaris Depletion.
