This technical note presents an optimized workflow that combines mRNA capture with globin and rRNA depletion to maximize sequencing efficiency from blood-derived RNA.
Highlights
- Maximize sequencing economy by combining mRNA capture with globin and rRNA depletion to focus reads on informative, coding transcripts.
- Enhance data quality with reduced duplication rates, increased gene detection, and uniform 5′ to 3′ transcript coverage across a range of RNA inputs.
- Streamline your workflow with a single-day protocol (~6.25 hours) optimized for high-yield, high-complexity RNA-seq libraries from blood samples.
