STELLARSCRIPT REVERSE TRANSCRIPTASE KITS

PRODUCTS
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StellarScript Highlights

  • Increased activity generates cDNA from exceedingly low RNA inputs
  • Template switching activity delivers compatibility with applications such as single-cell RNA-seq and 5’ RACE
  • Enhanced thermostability in comparison with wild-type enzyme improves reverse transcription through RNA secondary structure
  • Kits contain all required reagents for cDNA synthesis, except primers, for simple experimental setup
  • Custom formats available, including those to support lyophilization applications
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Applications

  • First strand cDNA synthesis
  • Reverse transcription quantitative PCR
    (RT-qPCR)
  • Reverse transcription PCR (RT-PCR)
  • Primer extension
  • RNA sequencing (RNA-seq)
  • Single-cell RNA sequencing (scRNA-seq)
  • 3’ and 5’ Rapid amplification of cDNA ends (RACE)
  • Applications leveraging SuperScript II for reverse transcription

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High-purity RNase Inhibitor

Protect what’s precious for your single-cell, single-nuclei, and RT-qPCR workflows.

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Key Performance Data

Achieve robust performance with low-input amounts

Many applications are limited in the available amount of input RNA, which can impact assay performance. StellarScript® generates high-quality cDNA across a wide range of RNA input amounts, delivering sensitive performance with very little RNA template.

Figure 1. StellarScript provides equivalent performance to SuperScript II. Watchmaker’s StellarScript and ThermoFisher Scientific’s SuperScript II were run in oligo-dT and randomly primed first strand synthesis at 42°C for 40 minutes using 1000 ng to 0.01 ng total liver RNA as template. Resulting cDNA yields were assessed via qPCR. StellarScript showed equivalency with inputs of 1 ng or more, and showed slight outperformance with lower input amounts.
Robust performance across inputs
Figure 1. StellarScript provides equivalent performance to SuperScript II. Watchmaker’s StellarScript and ThermoFisher Scientific’s SuperScript II were run in oligo-dT and randomly primed first strand synthesis at 42°C for 40 minutes using 1000 ng to 0.01 ng total liver RNA as template. Resulting cDNA yields were assessed via qPCR. StellarScript showed equivalency with inputs of 1 ng or more, and showed slight outperformance with lower input amounts.
Figure 1. StellarScript provides equivalent performance to SuperScript II. Watchmaker’s StellarScript and ThermoFisher Scientific’s SuperScript II were run in oligo-dT and randomly primed first strand synthesis at 42°C for 40 minutes using 1000 ng to 0.01 ng total liver RNA as template. Resulting cDNA yields were assessed via qPCR. StellarScript showed equivalency with inputs of 1 ng or more, and showed slight outperformance with lower input amounts.
Improve specificity with a thermostable RT

StellarScript provides enhanced thermostability in comparison to wild-type M-MLV RT and performs equivalent-to-better in comparison to other commercially available M-MLV RT variants with respect to both cDNA yield and lengths generated at elevated temperatures. This improves performance with RNAs that have high degrees of secondary structure, including viral RNA and those that are GC-rich.

For further enhanced thermostability, see StellarScript HT and StellarScript HT+.

Figure 2A. Improved performance at elevated temperatures. Watchmaker’s StellarScript, ThermoFisher Scientific’s SuperScript II, and Promega’s M-MLV Reverse Transcriptase, RNase H Minus were run in oligo-dT primed first strand synthesis at 42°C, 50°C or 55°C for 30 minutes using a 0.5 to 9 kb RNA ladder as template. StellarScript and SuperScript II have robust processivity at 42°C and superior processivity compared to Promega’s reverse transcriptase at elevated temperatures.
Figure 2B. Improved performance at elevated temperatures. Watchmaker’s StellarScript and ThermoFisher Scientific’s SuperScript II were run in oligo-dT-primed first strand synthesis at 42°C, 50°C, 60°C or 65°C for 25 minutes using 10 ng total liver RNA as template. Resulting cDNA mass was assessed via qPCR. StellarScript produced higher yields (indicated by lower Cq values) at any evaluated temperature than SuperScript II.
Improve specificity with a thermostable RT
Figure 2A. Improved performance at elevated temperatures. Watchmaker’s StellarScript, ThermoFisher Scientific’s SuperScript II, and Promega’s M-MLV Reverse Transcriptase, RNase H Minus were run in oligo-dT primed first strand synthesis at 42°C, 50°C or 55°C for 30 minutes using a 0.5 to 9 kb RNA ladder as template. StellarScript and SuperScript II have robust processivity at 42°C and superior processivity compared to Promega’s reverse transcriptase at elevated temperatures.
Figure 2A. Improved performance at elevated temperatures. Watchmaker’s StellarScript, ThermoFisher Scientific’s SuperScript II, and Promega’s M-MLV Reverse Transcriptase, RNase H Minus were run in oligo-dT primed first strand synthesis at 42°C, 50°C or 55°C for 30 minutes using a 0.5 to 9 kb RNA ladder as template. StellarScript and SuperScript II have robust processivity at 42°C and superior processivity compared to Promega’s reverse transcriptase at elevated temperatures.
Figure 2B. Improved performance at elevated temperatures. Watchmaker’s StellarScript and ThermoFisher Scientific’s SuperScript II were run in oligo-dT-primed first strand synthesis at 42°C, 50°C, 60°C or 65°C for 25 minutes using 10 ng total liver RNA as template. Resulting cDNA mass was assessed via qPCR. StellarScript produced higher yields (indicated by lower Cq values) at any evaluated temperature than SuperScript II.
Figure 2B. Improved performance at elevated temperatures. Watchmaker’s StellarScript and ThermoFisher Scientific’s SuperScript II were run in oligo-dT-primed first strand synthesis at 42°C, 50°C, 60°C or 65°C for 25 minutes using 10 ng total liver RNA as template. Resulting cDNA mass was assessed via qPCR. StellarScript produced higher yields (indicated by lower Cq values) at any evaluated temperature than SuperScript II.
For assay developers – make realizing your next product easier
  • Navigate the complexities of productization with an experienced team who’s as committed to your success as you are
  • All aspects of our customization process are designed to serve you with speed, agility, and above all else, a commitment to quality
  • Tailored fill volumes, labeling (including white and private label), and packaging designed to your specifications
  • All products are manufactured within an ISO 13485:2016-certified QMS (download our certificate)

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  Property Application
  Optimal
Temp		 Thermal
Stability		 Inhibitor
Tolerance		 Template
Switching		 RT-qPCR/
qRT-PCR		 RT-PCR RNA-seq scRNA-seq 5′ RACE 3′ RACE
StellarScript HT+ 42 – 65˚C +++ +++ + +++ +++ + + + +
StellarScript HT 42 – 50˚C ++ ++ – ++ ++ + – – +
StellarScript 42˚C + + + + + + + + +

Table 1. Overview of StellarScript portfolio enzyme properties and relevant applications. “+” signs indicate strength of a property or fit of an application for a specific enzyme.

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