Sensitivity meets speed
Transcriptomics provides a snapshot of cellular activity at specific points in time and in response to environmental stimuli. It aims to characterize both protein-coding and non-coding transcripts (and the variation therein) and quantify their complex ebb and flow. This allows researchers to unravel biological mechanisms, detect and monitor cancer and other diseases, and validate biomarkers and drug responses.
There is a need for RNA library preparation solutions that are highly sensitive, scalable, robust with respect to performance with challenging clinically relevant sample types, such as FFPE and low inputs. Our portfolio delivers in all of these areas, enabling cutting edge research and applications in the transcriptomics space.
- Watchmaker RNA Library Prep Kits with Polaris™ Depletion for improved sensitivity with FFPE and low-inputs using a rapid library prep workflow
- Watchmaker mRNA Library Prep Kits for library prep in < 5 hours with as little as 2.5 ng
- Watchmaker RNA Library Prep Kits for the streamlined prep of total RNA libraries for target enrichment applications
Key Highlights
- Increase sensitivity with FFPE and low-input samples
- Generate high-quality libraries with rapid, easily automated workflows
- Improve automatability with fewer cleanups and handling steps
- Discover the value of a reverse transcriptase engineered specifically for RNA sequencing
Rapid RNA-seq workflows
Figure 1. Truly single-day RNA library prep. The Watchmaker RNA portfolio offers three streamlined workflow options to address any RNA sequencing application and associated sample type. Reduced incubation times and fewer purification and handling steps makes all three workflows highly automatable and easily completed in a single workday.
Product comparison
| Watchmaker RNA Library Prep Kit with Polaris Depletion |
Watchmaker mRNA Library Prep Kits |
Watchmaker RNA Library Prep Kit |
|
| RNA enrichment method | Ribosomal and globin depletion | Bead-based poly(A) capture | None |
| RNA input | 1 to 1000 ng | 2.5 to 1000 ng | 0.25 to 100 ng |
| Library Prep Time | < 4.5 hours | < 5 hours | < 3.5 hours |
| Species compatibility | Human, mouse, and rat | Any eukaryote | Any |
| Sample type compatibility | FFPE Blood Tissue biopsies Dried blood spots* Cell lines and organoids Intact and degraded |
Intact (RIN > 7) Tissue biopsies Cell lines and organoids |
Any |
| Types of RNA detected | Protein-coding (exonic; mRNA) Long non-coding (lncRNA) |
Protein-coding (exonic mRNA) | Protein-coding (exonic; mRNA) Long non-coding (lncRNA) |
| Includes reagents for: | rRNA/globin depletion FFPE treatment RNA library prep Library amplification |
mRNA Capture RNA library prep Library amplification |
FFPE treatment RNA library prep Library amplification |
* From external testing
Application fit
| Watchmaker RNA Library Prep Kit with Polaris Depletion |
Watchmaker mRNA Library Prep Kits |
Watchmaker RNA Library Prep Kit |
|
| Gene expression analysis | X | X | |
| Gene fusion detection | X | X | X |
| Isoform/alternative splicing analysis | X | X | |
| Novel transcript discovery | X | X | |
| Single nucleotide variant (SNV) detection | X | X | X |
| Long non-coding RNA-seq | X | ||
| Targeted sequencing | X | ||
Learn more about:
Watchmaker DNA Library Prep Kits
A simple workflow that improves yields and coverage of low-input DNA samples, such as cfDNA.
Watchmaker DNA Library Prep Kits with Fragmentation
A streamlined single-tube chemistry with reduced bias and artifacts. Ideally suited for PCR-free workflows, ultra-low inputs, and low-quality DNA samples including FFPE
Equinox® Library Amplification Kits
An ultra-high-fidelity DNA polymerase in an optimized hot start master mix format, specifically optimized for high-efficiency, low-bias NGS library amplification