TAQ DNA POLYMERASE: Specifications

PRODUCTS
CONFIGURATION
Kit contents Taq DNA Polymerase (5 U/µL)
Unit definition One unit of Taq DNA polymerase is the amount of enzyme that will incorporate 10 nmol of dNTP into activated calf thymus DNA in 30 minutes at 75°C
Enzyme storage buffer 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.5% Tween
QUALITY CONTROL
Protein Purity Assay ≥ 99%
dsDNA Exonuclease Assay* <1% released
ssDNA Exonuclease Assay* <1% released
DNA contamination Assay (E. coli, mammalian, library)** < 10 copies
Phosphatase Contamination Assay* < 1% released
Endonuclease Contamination Assay* Not detectable

*As assessed using 37 U of enzyme input per assay.
**As assessed using 5 U of enzyme input per assay.

SHIPPING AND HANDLING
Shipping conditions Ice packs
Storage -20°C ± 5°C
Shelf life (off-the-shelf products) ≥ 12 months
Shelf life (custom products) ≥ 18 months