CONFIGURATION | |
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Kit contents | Taq DNA Polymerase (5 U/µL) |
Unit definition | One unit of Taq DNA polymerase is the amount of enzyme that will incorporate 10 nmol of dNTP into activated calf thymus DNA in 30 minutes at 75°C |
Enzyme storage buffer | 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.5% Tween |
QUALITY CONTROL | |
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Protein Purity Assay | ≥ 99% |
dsDNA Exonuclease Assay* | <1% released |
ssDNA Exonuclease Assay* | <1% released |
DNA contamination Assay (E. coli, mammalian, library)** | < 10 copies |
Phosphatase Contamination Assay* | < 1% released |
Endonuclease Contamination Assay* | Not detectable |
*As assessed using 37 U of enzyme input per assay.
**As assessed using 5 U of enzyme input per assay.
SHIPPING AND HANDLING | |
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Shipping conditions | Ice packs |
Storage | -20°C ± 5°C |
Shelf life (off-the-shelf products) | ≥ 12 months |
Shelf life (custom products) | ≥ 18 months |