T4 DNA POLYMERASE: Specifications

PRODUCTS
CONFIGURATION
Kit contents T4 DNA Polymerase (3 U/µL)
Unit definition One unit is defined as the amount of T4 DNA Polymerase that will incorporate 160 nmol of dNTPs into a DNA template in 60 minutes at 37°C
Storage buffer 100 mM Potassium Phosphate, pH 6.5, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol
QUALITY CONTROL
Protein Purity Assay ≥ 98%
Endonuclease Contamination Assay* Not detectable
DNA contamination Assay (E. coli, mammalian, library)* < 10 copies
Phosphatase Contamination Assay* < 1% released
Unit Assay* Pass

*As assessed using 2 U of enzyme input per assay.

SHIPPING AND HANDLING
Shipping conditions Ice packs
Storage -20°C ± 5°C
Shelf life (off-the-shelf products) ≥ 12 months
Shelf life (custom products) ≥ 18 months