STELLARTAQ
DNA POLYMERASE

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PCR. Uninhibited.

StellarTaq™ DNA Polymerase (DNAP) is engineered for extreme inhibitor tolerance, speed, and specificity. The polymerase catalyzes 5′ → 3′ DNA synthesis, has 5′ → 3′ exonuclease activity, and is deficient in 3′ → 5′ exonuclease activity making it suitable for probe digestion. It amplifies uracil-containing templates, incorporates modified bases, and performs A-tailing on DNA products. Available in hot start, non-hot start, and glycerol-free formats.

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Key Features and Benefits

  • Extreme inhibitor tolerance offers robust amplification across a range of clinical sample types, including urine, blood, sputum and bile
  • High speed enables fast PCR applications
  • Hot start mechanism ensures high specificity
  • Custom formats, including high concentrate and glycerol-free, support lyophilization
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ADLM 2024 poster: Purpose built reverse transcriptases and Taq DNA Polymerases deliver high yield in the presence of inhibitors

Applications

  • Pathogen detection, including infectious diseases
  • Fast PCR
  • RT-qPCR
  • PCR amplification of DNA fragments ≤ 5 kb
  • Probe and intercalating dye-based qPCR
  • PCR applications where biological inhibitors are present and specificity is important

Key Performance Data

Extreme inhibitor tolerance

Inhibitors – ubiquitous in biological samples – can interfere with amplification, leading to inaccurate results and false negatives. Using an inhibitor-tolerant polymerase ensures reliable amplification even in the presence of challenging substances found in clinically relevant samples, essential for accurate pathogen detection. StellarTaq delivers leading inhibitor-tolerance for accurate and reproducible results every time.

Figure 1. Enable amplification of challenging sample types with inhibitor-tolerant StellarTaq. A probe-based qPCR assay was employed in the presence of increasing inhibitors with wild-type (WT) Taq DNA Polymerase (DNAP), Competitor A engineered Taq, and StellarTaq DNAP. As the inhibitor cocktail concentration is increased, DNA yields decrease for WT Taq. In contrast, StellarTaq exhibits extreme inhibitor tolerance — retaining robust DNA amplification.
Extreme inhibitor tolerance
Figure 1. Enable amplification of challenging sample types with inhibitor-tolerant StellarTaq. A probe-based qPCR assay was employed in the presence of increasing inhibitors with wild-type (WT) Taq DNA Polymerase (DNAP), Competitor A engineered Taq, and StellarTaq DNAP. As the inhibitor cocktail concentration is increased, DNA yields decrease for WT Taq. In contrast, StellarTaq exhibits extreme inhibitor tolerance — retaining robust DNA amplification.
Figure 1. Enable amplification of challenging sample types with inhibitor-tolerant StellarTaq. A probe-based qPCR assay was employed in the presence of increasing inhibitors with wild-type (WT) Taq DNA Polymerase (DNAP), Competitor A engineered Taq, and StellarTaq DNAP. As the inhibitor cocktail concentration is increased, DNA yields decrease for WT Taq. In contrast, StellarTaq exhibits extreme inhibitor tolerance — retaining robust DNA amplification.
Enable fast PCR

Fast PCR is essential for pathogen detection, providing rapid identification of contaminating bacteria and viruses. Its reduced reaction times enhance workflow efficiency, boost lab throughput, and enable timely decision-making.

Figure 2. High polymerization speeds support fast PCR. StellarTaq DNA Polymerase (DNAP), Wild-type Taq DNAP, and a variety of engineered Taq DNAPs (Competitors A, B, and C) were evaluated for initial velocity – taken as a representation of polymerization speed – wherein a pre-primed, defined ssDNA template was extended by each polymerase at 72°C and intercalating dye fluorescence was measured over time. StellarTaq delivered the highest polymerization speed out of all enzymes compared.
Enable fast PCR
Figure 2. High polymerization speeds support fast PCR. StellarTaq DNA Polymerase (DNAP), Wild-type Taq DNAP, and a variety of engineered Taq DNAPs (Competitors A, B, and C) were evaluated for initial velocity – taken as a representation of polymerization speed – wherein a pre-primed, defined ssDNA template was extended by each polymerase at 72°C and intercalating dye fluorescence was measured over time. StellarTaq delivered the highest polymerization speed out of all enzymes compared.
Figure 2. High polymerization speeds support fast PCR. StellarTaq DNA Polymerase (DNAP), Wild-type Taq DNAP, and a variety of engineered Taq DNAPs (Competitors A, B, and C) were evaluated for initial velocity – taken as a representation of polymerization speed – wherein a pre-primed, defined ssDNA template was extended by each polymerase at 72°C and intercalating dye fluorescence was measured over time. StellarTaq delivered the highest polymerization speed out of all enzymes compared.
For assay developers – make realizing your next product easier
  • Navigate the complexities of productization with an experienced team who’s as committed to your success as you are
  • All aspects of our customization process are designed to serve you with speed, agility, and above all else, a commitment to quality
  • Tailored fill volumes, labeling (including white and private label), and packaging designed to your specifications
  • All products are manufactured within an ISO 13485:2016-certified QMS (download our certificate)
QC Specifications
Description Specification
Protein Purity Assay ≥ 97%
RNase Contamination Assay* Not detectable
Endonuclease Contamination Assay* Not detectable
DNA contamination Assay (E. coli, mammalian, library) < 10 copies
dsDNA Exonuclease Assay* < 1% released
ssDNA Exonuclease Assay* < 1% released
Phosphatase Contamination Assay* < 1% released

*As assessed using 50 U of protein input per assay.

Properties
Unit definition: One unit of StellarTaq DNA Polymerase incorporates 16 nmol of dNTPs into a DNA template in 30 minutes at 72°C

Reaction conditions* (materials not included in this kit):

  • 20 mM Tris-HCl, pH 8.3
  • 100 mM KCl
  • 0.004% Tween 20

*Baseline reaction condition, optimization is required. See technical guide for more information.

Enzyme storage buffer:

  • Standard: 50 mM Tris-HCl, pH 7.5, 100 mM KCl, 0.1 mM EDTA, 50% Glycerol, 0.05% Tween 20
  • Glycerol-free: 50 mM Tris-HCl, pH 7.5, 300 mM KCl, 0.05% Tween 20
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