Consistently Reliable Isothermal Amplification
The need for fast, field-deployable molecular assays has never been greater. Recombinase Polymerase Amplification (RPA) is a highly sensitive isothermal amplification method that enables rapid, onsite pathogen testing, including for infectious diseases. However, enzyme and protein lot-to-lot variability has historically limited its reliability – until now.
Key Features and Benefits
- Amplify consistently with individually QC’d enzymes and proteins, improving performance and reproducibility
- Overcome LAMP limitations with lower amplification temperatures, simple primer design, and shorter amplification times
- Go mobile with glycerol-free, lyophilization-friendly formulations, enabling robust field-based testing
Figure 1. Molecular representation of Recombinase Polymerase Amplification (RPA). T4 UvsX recombinase (green ovals) and UvsY protein (purple hexagons) bind to amplification primers, forming a complex that is complementary to sequences in double stranded DNA. T4 gp32 protein (pink dots) acts as a single-stranded binding protein and stabilizes the unwound DNA strand, allowing Bsu DNA Polymerase – Large Fragment (red ovals) to initiate strand displacing amplification. The repetition of the cycle leads to exponential amplification. Schematic recreated from James, et al, Diagnostics 2020, 10, 399.
Proteins Available
| Protein | Role |
|---|---|
| T4 UvsX Recombinase (glycerol -) |
Quality controlled for enzyme activity (forming D-loop recombination structures for initiation of amplification) and freeze/thaw stable |
| T4 UvsY Protein (glycerol -) |
Accessory protein to T4 UvsX Recombinase with robust single stranded binding |
| T4 Gene 32 Protein (glycerol +/-) |
Stabilizes ssDNA to increase amplification efficiency and primer specificity |
| Bsu DNA Polymerase, Large Fragment (glycerol +/-) |
Displays strong strand displacement and enables isothermal amplification at lower temperatures than Bst polymerase |
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