RNase INHIBITOR

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RNase Inhibitor exhibits high affinity, non-competitive binding of RNases A, B, and C at a 1:1 ratio, enabling high-quality cDNA synthesis from low-quality RNA samples. The absence of two cysteines present in human and porcine RNase inhibitors make this murine version highly suitable for low-DTT applications.

  • Prevent RNA degradation in RT-qPCR, single cell, and single nuclei sequencing workflows at temperatures up to 55°C
  • Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price-point
  • Improve performance in low-DTT settings with a murine RNase inhibitor that better resists oxidation
  • Custom formats, including high concentration, support lyophilization applications
  • Highly stringent enzyme manufacturing ensures quality performance across lots
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StellarScript HT+ Reverse Transcriptase

Highly thermostable and inhibitor-tolerant for improved performance in RT-PCR and RT-qPCR applications

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Key Performance Data

Safeguard RNA assay performance

Ribonucleases (RNases) are ubiquitous and can have significant and detrimental impacts on assay performance and sensitivity. Incorporating an RNase inhibitor to prevent RNA degradation ensures reliability and accuracy of experimental results – particularly for studies and assays focused on pathogen detection, gene expression, RNA stability, and other RNA-dependent processes.

Figure 1A. Prevent RNA degradation through the addition of RNase inhibitor. The addition of RNase inhibitor to first strand synthesis (FSS) reactions improves cDNA yield even when no RNase A is added to the reaction — highlighting risks of RNase contamination during sample handling. Addition of 5 ng RNase A results in a >10-fold decrease in cDNA yield when no RNase inhibitor is present. Assay performance is rescued by the addition of RNase inhibitor, where all commercial enzymes evaluated perform equivalently. RNase Inhibitors sourced from a variety of vendors were added to oligo-dT-primed FSS reactions with StellarScript® HT+ Reverse Transcriptase. Reactions ran for 30 min at 42°C using 10 ng total liver RNA.
Figure 1B. Prevent RNA degradation at elevated temperatures through the addition of RNase Inhibitor. The addition of RNase Inhibitor to high-temperature (55°C) first strand synthesis (FSS) reactions improves cDNA yield even when no RNase A is added to the reaction — highlighting risks of RNase contamination during sample handling. Addperformance is rescued by the addition of RNase Inhibitor. RNition of 5 ng RNase A results in a >10-fold decrease in cDNA yield when no RNase Inhibitor is present. Assay ase Inhibitor was added to oligo-dT-primed FSS reactions with StellarScript HT+ Reverse Transcriptase. Reactions ran for 30 min at 55°C using 10 ng total liver RNA.
Safeguard RNA assay performance
Figure 1A. Prevent RNA degradation through the addition of RNase inhibitor. The addition of RNase inhibitor to first strand synthesis (FSS) reactions improves cDNA yield even when no RNase A is added to the reaction — highlighting risks of RNase contamination during sample handling. Addition of 5 ng RNase A results in a >10-fold decrease in cDNA yield when no RNase inhibitor is present. Assay performance is rescued by the addition of RNase inhibitor, where all commercial enzymes evaluated perform equivalently. RNase Inhibitors sourced from a variety of vendors were added to oligo-dT-primed FSS reactions with StellarScript® HT+ Reverse Transcriptase. Reactions ran for 30 min at 42°C using 10 ng total liver RNA.
Figure 1A. Prevent RNA degradation through the addition of RNase inhibitor. The addition of RNase inhibitor to first strand synthesis (FSS) reactions improves cDNA yield even when no RNase A is added to the reaction — highlighting risks of RNase contamination during sample handling. Addition of 5 ng RNase A results in a >10-fold decrease in cDNA yield when no RNase inhibitor is present. Assay performance is rescued by the addition of RNase inhibitor, where all commercial enzymes evaluated perform equivalently. RNase Inhibitors sourced from a variety of vendors were added to oligo-dT-primed FSS reactions with StellarScript® HT+ Reverse Transcriptase. Reactions ran for 30 min at 42°C using 10 ng total liver RNA.
Figure 1B. Prevent RNA degradation at elevated temperatures through the addition of RNase Inhibitor. The addition of RNase Inhibitor to high-temperature (55°C) first strand synthesis (FSS) reactions improves cDNA yield even when no RNase A is added to the reaction — highlighting risks of RNase contamination during sample handling. Addperformance is rescued by the addition of RNase Inhibitor. RNition of 5 ng RNase A results in a >10-fold decrease in cDNA yield when no RNase Inhibitor is present. Assay ase Inhibitor was added to oligo-dT-primed FSS reactions with StellarScript HT+ Reverse Transcriptase. Reactions ran for 30 min at 55°C using 10 ng total liver RNA.
Figure 1B. Prevent RNA degradation at elevated temperatures through the addition of RNase Inhibitor. The addition of RNase Inhibitor to high-temperature (55°C) first strand synthesis (FSS) reactions improves cDNA yield even when no RNase A is added to the reaction — highlighting risks of RNase contamination during sample handling. Addperformance is rescued by the addition of RNase Inhibitor. RNition of 5 ng RNase A results in a >10-fold decrease in cDNA yield when no RNase Inhibitor is present. Assay ase Inhibitor was added to oligo-dT-primed FSS reactions with StellarScript HT+ Reverse Transcriptase. Reactions ran for 30 min at 55°C using 10 ng total liver RNA.
QC Specifications
Description Specification
Protein Purity Assay ≥ 97%
dsDNA Exonuclease Assay* <1% released
ssDNA Exonuclease Assay* <1% released
DNA contamination Assay (E. coli, mammalian, library)* < 10 copies
Phosphatase Contamination Assay* < 1% released
Endonuclease Contamination Assay* Not detectable
Nonspecific RNase* Not detectable

*As assessed using 450 U of protein input per assay.

Properties

Unit definition: One unit of RNase inhibitor is defined as the amount of RNase Inhibitor required to inhibit activity of 0.375 ng of RNase A by ≥ 95%

Reaction conditions:
Active at temperatures ≤ 55°C

Storage buffer: 20 mM HEPES-KOH, 0.1 mM EDTA, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.6

Heat inactivation: 70°C for 20 min

Molecular weight: 50.6 kDa

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