What is T4 Gene 32 Protein (T4 gp32)?
T4 gp32 is a specific single strand DNA binding protein that is involved in T4 bacteriophage replication and repair. T4 gp32 binds and stabilizes single stranded DNA and is utilized as an additive in molecular biology assays to increase DNA yield.
What are the applications for T4 gp32?
- PCR
- Electron microscopy
- Restriction enzyme digests
- RT-PCR
- Whole genome amplification (WGA)
- Helicase-dependent amplification
What is the storage buffer for T4 gp32?
T4 gp32 storage buffer is 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 50% Glycerol.
How do we assess concentration of T4 gp32?
T4 gp32 is supplied at a concentration of 10 mg/mL. Concentration is assessed by nanodrop (DeNovix), blanked using the storage buffer and an extinction coefficient of E1% 11.76 g/100 mL.
How do we assess the functionality of T4 gp32?
The ssDNA binding affinity of T4 gp32 (reported as Kd) is determined by incubating a dilution series of T4 gp32 with a constant amount of fluorescently labeled ssDNA. The resulting signal change in fluorescence polarization is used to determine the binding affinity (Kd).
What is the extinction coefficient for T4 gp32?
The extinction coefficient of E1% is 11.76 g/100mL.
What are the recommended reaction setup conditions?
Please reference our technical guide for instructions for use.
Do you offer custom formats?
Yes! Watchmaker offers customer fills, packaging, concentrations, and labeling – including private label – designed to meet your unique needs. We offer flexible terms to serve organizations of any size, and our right-sized processes enable rapid turnaround time on customization. Please contact sales@watchmakergenomics.com to learn more about our capabilities.
Are you ISO 13485-certified?
Yes! Our Quality Management System has achieved ISO 13485:2016 Certification. Our certificate has been awarded for the design, development, manufacture, contract manufacture, and support of high performing reagents for genomics applications in medical research. Download the certificate here.