| CONFIGURATION | |
|---|---|
| Kit contents | DNA Polymerase I (10 U/µL) |
| Unit definition | One unit is defined as the amount of DNA Polymerase I that will incorporate 300 nmol of dNTPs into a DNA template in 60 minutes at 37°C |
| Enzyme storage buffer | 25 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 50% Glycerol, 1 mM DTT |
| QUALITY CONTROL | |
|---|---|
| Protein Purity Assay | ≥ 99% |
| Endonuclease Contamination Assay* | Not detectable |
| DNA contamination Assay (E. coli, mammalian, library)* | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Unit Assay* | Pass |
*As assessed using 2 U of enzyme input per assay.
| SHIPPING AND HANDLING | |
|---|---|
| Shipping conditions | Ice packs |
| Storage | -20°C ± 5°C |
| Shelf life (off-the-shelf products) | ≥ 12 months |
| Shelf life (custom products) | ≥ 18 months |