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DNA Polymerase I Highlights
DNA Polymerase I is a mesophilic E. coli polymerase that catalyzes 5′ → 3′ template-directed DNA synthesis. DNA Polymerase I has 3′ → 5′ exonuclease (proofreading) activity and 5′ → 3′ exonuclease activity. It is capable of catalyzing de novo synthesis of synthetic homopolymers and provides a convenient method for the preparation of a variety of defined DNA substrates in the laboratory.
- Effective nick translation
- Reduce material costs with a drop-in solution that delivers equivalent performance at a differentiated price-point
- Custom formats, including high concentration, support lyophilization applications
- Highly stringent enzyme manufacturing ensures quality performance across lots
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Applications
- Nick translation
- Second-strand synthesis
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High-purity T4 DNA Ligase
Available with standard and rapid ligation buffers for simple optimization
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- All aspects of our customization process are designed to serve you with speed, agility, and above all else, a commitment to quality
- Tailored fill volumes, labeling (including white and private label), and packaging designed to your specifications
- All products are manufactured within an ISO 13485:2016-certified QMS (download our certificate)
QC Specifications
| Description | Specification |
|---|---|
| Protein Purity Assay | ≥ 99% |
| Endonuclease Contamination Assay* | Not detectable |
| DNA contamination Assay (E. coli, mammalian, library)* | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Unit Assay* | Pass |
*As assessed using 2 U of enzyme input per assay.
Properties
Unit definition: One unit is defined as the amount of DNA Polymerase I that will incorporate 300 nmol of dNTPs into a DNA template in 60 minutes at 37°C
Reaction conditions (materials not included in the kit):
50 mM Tris-HCl, pH 7.9
10 mM MgCl₂
1 mM DTT
Storage buffer: 25 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 50% Glycerol, 1 mM DTT
Heat inactivation: 75°C for 20 min
Molecular weight: 103 kDa
5′ – 3′ Exonuclease Activity: Yes
3′ – 5′ Exonuclease Activity: Yes
Strand Displacement Activity: No
Fidelity: High
Processivity: Moderate