| CONFIGURATION | |
|---|---|
| Kit contents (depending on product ordered) |
StellarTaq™ Hot Start DNA Polymerase (5 U/µL) StellarTaq Hot Start DNA Polymerase - Glycerol-Free (30 U/uL or 140 U/µL) StellarTaq DNA Polymerase (5 U/µl) StellarTaq DNA Polymerase - Glycerol-Free (140 U/µL) |
| Unit definition | One unit of StellarTaq DNA Polymerase incorporates 16 nmol of dNTPs into a DNA template in 30 minutes at 72°C. |
| Enzyme storage buffer |
Standard: 50 mM Tris-HCl, pH 7.5, 100 mM KCl, 0.1 mM EDTA, 50% Glycerol, 0.05% Tween 20 Glycerol-free: 50 mM Tris-HCl, pH 7.5, 300 mM KCl, 0.05% Tween 20 |
| QUALITY CONTROL | |
|---|---|
| Protein Purity Assay | ≥97% |
| RNase Contamination Assay* | Not detectable |
| Endonuclease Contamination Assay* | Not detectable |
| DNA Contamination Assay (E. coli, mammalian, library) |
<10 copies |
| dsDNA Exonuclease Contamination Assay* | <1% released |
| ssDNA Exonuclease Contamination Assay* | <1% released |
| Phosphatase Contamination Assay* | <1% release |
| Unit Assay | Passed |
| * As assessed using 50 U of protein input per assay | |
| SHIPPING AND HANDLING | |
|---|---|
| Shipping conditions |
Glycerol-containing versions: Ice packs Glycerol-free versions: Dry Ice |
| Storage | -20°C ± 5°C |
| Shelf life | 12 months |