| CONFIGURATION | |
|---|---|
| Kit contents | phi29 DNA Polymerase (10 U/µL or 100 U/µL), 10X phi29 Pol Reaction Buffer (buffer not included in 100 U/µL kit) |
| Unit definition | One unit is defined as the amount of enzyme required to convert 50 pmol of dNTPs into a polynucleotide fraction in 10 minutes at 30°C. |
| Enzyme storage buffer | 10 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% Glycerol, 0.5% Tween |
| 10X phi29 Pol Reaction Buffer | 330 mM Tris-acetate (pH 8.2 at 25°C), 100 mM Mg-acetate, 660 mM K-acetate, 1% (v/v) Tween 20, 10 mM DTT |
| QUALITY CONTROL | |
|---|---|
| Protein Purity Assay | ≥ 99% |
| Exonuclease Assay | Functional |
| Specific Activity | 100,000 – 190,000 U/mg |
| DNA contamination Assay (E. coli, mammalian, library)* | < 10 copies |
| Phosphatase Contamination Assay* | < 1% released |
| Endonuclease Contamination Assay* | Not detectable |
*As assessed using 1,000 U of enzyme input per assay.
| SHIPPING AND HANDLING | |
|---|---|
| Shipping conditions | Ice packs |
| Storage | -20°C ± 5°C |
| Shelf life (off-the-shelf products) | 12 months |
| Shelf life (custom products) | ≥ 18 months |