phi29 DNA POLYMERASE: Specifications

Kit contents phi29 DNA Polymerase (10 U/µL or 100 U/µL), 10X phi29 Pol Reaction Buffer (buffer not included in 100 U/µL kit)
Unit definition One unit is defined as the amount of enzyme required to convert 50 pmol of dNTPs into a polynucleotide fraction in 10 minutes at 30°C.
Enzyme storage buffer 10 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% Glycerol, 0.5% Tween
10X phi29 Pol Reaction Buffer 330 mM Tris-acetate (pH 8.2 at 25°C), 100 mM Mg-acetate, 660 mM K-acetate, 1% (v/v) Tween 20, 10 mM DTT
Protein Purity Assay ≥ 99%
Exonuclease Assay Functional
Specific Activity 100,000 – 190,000 U/mg
DNA contamination Assay (E. coli, mammalian, library)* < 10 copies
Phosphatase Contamination Assay* < 1% released
Endonuclease Contamination Assay* Not detectable

*As assessed using 1,000 U of enzyme input per assay.

Shipping conditions Ice packs
Storage -20°C ± 5°C
Shelf life (off-the-shelf products) 12 months
Shelf life (custom products) ≥ 18 months